Macrolide Therapy in Respiratory Viral Infections

Background. Macrolides have received considerable attention for their anti-inflammatory and immunomodulatory actions beyond the antibacterial effect. These two properties may ensure some efficacy in a wide spectrum of respiratory viral infections. We aimed to summarize the properties of macrolides and their efficacy in a range of respiratory viral infection. Methods. A search of electronic journal articles through PubMed was performed using combinations of the following keywords including macrolides and respiratory viral infection. Results. Both in vitro and in vivo studies have provided evidence of their efficacy in respiratory viral infections including rhinovirus (RV), respiratory syncytial virus (RSV), and influenza virus. Much data showed that macrolides reduced viral titers of RV ICAM-1, which is the receptor for RV, and RV infection-induced cytokines including IL-1β, IL-6, IL-8, and TNF-α. Macrolides also reduced the release of proinflammatory cytokines which were induced by RSV infection, viral titers, RNA of RSV replication, and the susceptibility to RSV infection partly through the reduced expression of activated RhoA which is an RSV receptor. Similar effects of macrolides on the influenza virus infection and augmentation of the IL-12 by macrolides which is essential in reducing virus yield were revealed. Conclusion. This paper provides an overview on the properties of macrolides and their efficacy in various respiratory diseases

Comparison of the Plasma Metabolome Profiles Between the Internal Thoracic Artery and Ascending Aorta in Patients Undergoing Coronary Artery Bypass Graft Surgery Using Gas Chromatography Time-of-Flight Mass Spectrometry.

BackgroundThe left internal thoracic artery (LITA) has been used as the first conduit of choice in coronary artery bypass grafting (CABG) because of excellent long-term patency and outcomes. However, no studies have examined substances other than nitric oxide that could be beneficial for the bypass conduit, native coronary artery or ischemic myocardium. This study was conducted to evaluate differences in metabolic profiles between the LITA and ascending aorta using gas chromatography-time of flight-mass spectrometry (GC-TOF-MS).MethodsTwenty patients who underwent CABG using the LITA were prospectively enrolled. Plasma samples were collected simultaneously from the LITA and ascending aorta. GC-TOF-MS based untargeted metabolomic analyses were performed and a 2-step volcano plot analysis was used to identify distinguishable markers from two plasma metabolome profiles. Semi-quantitative and quantitative analyses were performed using GC-TOF-MS and enzyme-linked immunosorbent assay, respectively, after selecting target metabolites based on the metabolite set enrichment analysis.ResultsInitial volcano plot analysis demonstrated 5 possible markers among 851 peaks detected. The final analysis demonstrated that the L-cysteine peak was significantly higher in the LITA than in the ascending aorta (fold change = 1.86). The concentrations of intermediate metabolites such as L-cysteine, L-methionine and L-cystine in the 'cysteine and methionine metabolism pathway' were significantly higher in the LITA than in the ascending aorta (2.0-, 1.4- and 1.2-fold, respectively). Quantitative analysis showed that the concentration of hydrogen sulfide (H₂S) was significantly higher in the LITA.ConclusionThe plasma metabolome profiles of the LITA and ascending aorta were different, particularly higher plasma concentrations of L-cysteine and H₂S in the LITA

Contingent convertible bonds with the default risk premium

Contingent convertible bonds (CoCos) are hybrid instruments characterized by both debt and equity. CoCos are automatically converted into equity or written down when a predefined trigger event occurs. The present study quantifies the issuing bank's default risk that only manifests in the post-conversion period for pricing CoCos depending on a loss-absorbing method. This work aims to reflect the distinct features of equity-conversion CoCos - in contrast to a write-down CoCos - in a valuation framework. Accordingly, we propose a model to compute the ratio of common equity Tier 1 (CET1), which is composed of core capital and risky assets, by employing a geometric Brownian motion and a random variable. Then, we formulate the post-conversion risk premium by measuring the probability with which the bank's CET1 ratio breaches a regulatory default threshold after conversion. Finally, we empirically examine a positive value of the post-conversion risk premium embedded in the market prices of equity-conversion CoCos

Vav1 inhibits RANKL-induced osteoclast differentiation and bone resorption

Vav1 is a Rho/Rac guanine nucleotide exchange factor primarily expressed in hematopoietic cells. In this study, we investigated the potential role of Vav1 in osteoclast (OC) differentiation by comparing the ability of bone marrow mononuclear cells (BMMCs) obtained from Vav1-deficient (Vav1−/−) and wild-type (WT) mice to differentiate into mature OCs upon stimulation with macrophage colony stimulating factor and receptor activator of nuclear kappa B ligand in vitro. Our results suggested that Vav1 deficiency promoted the differentiation of BMMCs into OCs, as indicated by the increased expression of tartrate-resistant acid phosphatase, cathepsin K, and calcitonin receptor. Therefore, Vav1 may play a negative role in OC differentiation. This hypothesis was supported by the observation of more OCs in the femurs of Vav1−/− mice than in WT mice. Furthermore, the bone status of Vav1−/− mice was analyzed in situ and the femurs of Vav1−/− mice appeared abnormal, with poor bone density and fewer number of trabeculae. In addition, Vav1-deficient OCs showed stronger adhesion to vitronectin, an αvβ3 integrin ligand important in bone resorption. Thus, Vav1 may inhibit OC differentiation and protect against bone resorption

Purification and characterization of microbial protease produced extracellularly from Bacillus subtilis FBL-1

An ammonium sulfate precipitation of fermentation broth produced by Bacillus subtilis FBL-1 resulted in 2.9-fold increase of specific protease activity. An eluted protein fraction from the column chromatographies using DEAE-Cellulose and Sephadex G-75 had 94.2- and 94.9-fold higher specific protease activity, respectively. An SDS-PAGE revealed a band of purified protease at approximately 37.6 kDa. Although purified protease showed the highest activity at 45°C and pH 9.0, the activity remained stable in temperature range from 30 to 50°C and pH range from 7.0 to 9.0. Protease activity was activated by metal ions such as Ca2+, Mg2+, Mn2+, Fe2+, Ca2+ and K+, but 10 mM Fe3+ significantly inhibited enzyme activity (53%). Protease activity was inhibited by 2 mM EDTA as a metalloprotease inhibitor, but it showed good stability against surfactants and organic solvents. The preferred substrates for protease activity were found to be casein (100%) and soybean flour (71.6%)

ICE HOCKEY DATABASE SCHEMA DESIGN: FOR NATIONAL TEAM\u27S BIOMECHANICAL ANALYSIS

This study presented database schema to manage ice hockey data shown in official record of World Championships. We selected data fields to design database schema considering which fields were contributing to the victory. We also sorted all fields into six tables to reflect the elements of ice hockey games; Game Information, Team Information, Offensice Stats, Defence Stats, Face-off Stats, Time On Ice Stats. This study was a prior research designing database schema in which the analysis of ice hockey official record is used to advance from statistical models to data science techniques. After storing raw data pre-processed into the database, researcher can analyze biomechanically to improve performance

Effects of aiming lines and visual function on the golf putting alignment

Background: In golf, a player hits a ball with a club, aiming to transfer the ball successively into a series of hole cups in a course consisting of 18 (or fewer) holes. This study aimed to compare the impact of visual function and the presence and number of aiming lines on golf putting alignment between beginner and expert golfers. Methods: In this prospective comparative study, 43 participants with a mean ± standard deviation (SD) of corrected distance binocular visual acuity of –0.07 ± 0.74 logarithm of the minimum angle of resolution, who knew their average golf scores, were divided into beginner and expert golfers. Six visual function tests were conducted to assess heterotropia, dominant eye, verification of current spectacles, static visual acuity, stereopsis, and fixation disparity. At the putting distances of 1.5 m and 3 m, alignment errors were measured five times each, using golf balls with 1 and 3 aiming line(s) and putters with 1 and 3 aiming line(s). Results: The mean ± SD of age was 48.33 ± 10.07 years for study participants overall. The accuracy of ball alignment was not affected by the career or number of aiming lines, but the putter alignment was higher for the 3-lines putter than for the 1-line putter (P < 0.05). When the number and shape of the aiming line were the same for both the ball and putter, the aiming accuracy was found to be higher. In both stereopsis and fixation disparity, the combination of putting distance and a 3-lines ball showed negative values; all other combinations showed positive values, but no statistically significant correlation was detected (all P > 0.05). Conclusions: The accuracy of golf ball alignment did not depend on the number of aiming lines and the golfer’s career. However, the predicted putting success rate and subjective satisfaction were increased when three-line golf balls and putters were used, as compared to when one-line golf balls and putters were used. How to cite this article: Kim YJ, Jin YG, Koo BY, Jang JU, Mah KC. Effects of aiming lines and visual function on the golf putting alignment. Med Hypothesis Discov Innov Optom.2021 Spring; 2(1): 41-49. DOI: https://doi.org/10.51329/mehdioptometry12